The full form of the **ANOVA** is **Analysis of Variance**. This test helps us to identify whether there is a similarity in values between two data samples. or whether one dataset is significant compared to the other. The two-way**ANOVA** analysis means there should be involvement of two separate variables, and how they interact with each other. If you are curious to know how you can analyze two-way **ANOVA** in Excel with unequal sample sizes, then this article may come in handy for you. In this article, we will discuss how you can analyze two-way **ANOVA** in Excel with an unequal sample sizeÂ with elaborate explanations.

## Overview of **ANOVA** Analysis

**ANOVA** provides the first opportunity to determine which factors have a significant effect on a given set of data. After the analysis is completed, an analyst does extra analysis on the methodological factors that significantly impact the inconsistent nature of data sets. And he uses the **ANOVA** analysis findings in the **F-test** in order to create extra data relevant to the estimated regression analysis. The **ANOVA** analysis compares many data sets simultaneously to see whether there is a link between them. **ANOVA** is a statistical method used to analyze variance observed within a dataset by dividing it into two sections: 1) Systematic factors and 2) Random factors

The Formula of **ANOVA**:

`F= MSE / MST`

Here:

**F** = ANOVA coefficient

**MST** = Mean sum of squares due to treatment

**MSE** = Mean sum of squares due to error

**ANOVA** is of two types: single factor and two factors. The method is related to variance analysis.

- In two factors, there are multiple dependent variables and in one factor, there will be one dependent variable.
- Single-factor
**ANOVA**calculates the effect of one single factor on a single variable. And it checks whether all the**sample**data sets are of same or not. - Single-factor
**ANOVA**identifies the differences that are statistically significant between the average means of numerous variables.

## Two Way **ANOVA** with **Unequal** Sample Size in Excel: 2 Suitable Examples

We are going to do variance analysis by following the method of two factors without replication of **ANOVA** Analysis. Furthermore, We have some data on different exam scores of a school.Â Additionally, we have some data on the work hours of men and women. Both of them actually have unequal sample sizes. You want to do a **Data Analysis** of the ready data to find a relation between two groups in each example. Letâ€™s walk through the steps to do a two-way **ANOVA** analysis without replication with unequal sample sizes.

### Example 1: Two Way **ANOVA** for Student Marks

In the following example, we are going to use the dataset where we have the student’s marks in two separate groups. One group is **Early Riser** and another one is **Late Riser. **And the first group is one row short compared to the second group. We will apply **ANOVA** analysis to these two groups to see how they interact.

**Steps**

- In the beginning, we need to first add
**Data Analysis ToolPak.**Adding this toolpak will allow us to**ANOVA**analysis in two ways. - To add the
**Data Analysis Toolpak**, click on the**File**in the corner of the worksheet.

- Then from the
**Excel Options**dialog box, click on the**Add-ins**. - Right after this, from the
**Manage**option, choose**Excel Add-ins.** - Click
**Go**after this.

- In the next
**Add-ins**dialog box, tick the**Analysis ToolPak**checkbox.

- Now you can see that the
**Data Analysis**command is now present in the**Data**tab. - Click on the
**Data Analysis**command.

- In the
**Data Analysis**dialog box, select**ANOVA: Two-Factor With Replication**and then click on**OK**.

- After clicking
**OK**, you can see that there is a new dialog box, named**ANOVA: Two-Factor Without Replication**. In that dialog box, you need to select the input data for**ANOVA**calculations. - Select the range of cell
**B4:E12**in the**Input Range.** - Tick the
**Labels**check mark. - Now select the proper alpha value for the analysis. We are going for the usual 0.05 here.
- And select cell
**$G$2**, for the selection for the**Output Range**. - Click
**OK**after this.

- After clicking on
**OK**, the**ANOVA**two factor with replication result will appear on a new or already existing Excel spreadsheet depending on the option you have selected.

### Example 2: Two Way **ANOVA** for Working Hours

In the following example, we are going to use the dataset where we have the working hour in two separate groups. One group is for the Man and another one is for Women**. **And the first group is one row short compared to the second group. We will apply **ANOVA** analysis to these two groups to see how they interact.

**Steps**

- You can see that the
**Data Analysis**command is present in the**Data**tab. - Click on the
**Data Analysis**command.

- In the
**Data Analysis**dialog box, select**ANOVA: Two-Factor Without Replication**and then click on**OK**.

- After clicking
**OK**, you can see that there is a new dialog box, named**ANOVA: Two-Factor Without Replication**. In that dialog box, you need to select the input data for**ANOVA**calculations. - Select the range of cells
**B4:E12**in the**Input Range.** - Tick the
**Labels**check mark. - Now select the proper alpha value for the analysis. We are going for the usual 0.05 here.
- And select cell
**$G$2**, for the selection for the**Output Range**. - Click
**OK**after this.

- After clicking on
**OK**, the**ANOVA**two-factor without replication result will appear on a new or already existing Excel spreadsheet depending on the option you have selected.

**Interpretation of the Result**

The interpretation of any** ANOVA** analysis involves the Null hypothesis test. The **Null hypothesis** is that no comparing group is significantly different from the other group. If **F **> **F _{critical }**then we can reject the Null hypothesis. But on the other hand, if

**F**<

**F**, then we canâ€™t reject the

_{critical }**Null Hypothesis**, meaning that no comparing group is significantly different from any other group.

- In the first example, we can see that in both cases,
**F**<**F**. This means we canâ€™t reject the null hypothesis in those cases. Subsequently, this also means that there are strong similarities between the datasets._{critical} - In the second example, we can see that in row cases,
**F**<**F**. This means we canâ€™t reject the null hypothesis in those cases. Subsequently, this also means that there are strong similarities between the row of the datasets. But in column cases_{critical}**F**>**F**, which means that we can reject the Null hypothesis there. Subsequently, this also means that there arenâ€™t any strong similarities between the datasets in column sections._{critical} - We can also see that the P-value in the first example is 0.037 which is statistically significant so you can say that there is an effect of shifts on the performance of the students in the exam. But the value is close to the alpha value of 0.05 so the effect is less significant.
- The
**P value**in the first example is 0.510. So you can say that the working hour of the students have some significant impact on the marks they get. But as this value is not very close to the value of alpha, the effect is substantial. Meaning this has a heavy impact on the performances. - The P value in the first example is 0.0346. So you can say that there is an impact of gender in the distribution of workhour. But as this value is very close to the value of alpha, the effect is non-substantial. This means this gender impact is not very effective.

## Limitations of Two Way **ANOVA** Analysis with **Unequal** Sample Size in Excel

In this example, we showed how you could execute the **ANOVA **analysis with an unequal sample size. But there is a couple of restriction on calculating the **ANOVA**Â analysis with these specific criteria.

- There are no criteria like having the same equal sample sizes for
**ANOVA**calculation. So theoretically this**ANOVA**analysis with unequal sample sizesÂ can be done for both one-way and two-way analyses. - But there is a small rule. The variance difference between the
**sample**datasets must be close. If the unevenness of**sample****sizes**between the dataset is too big, then**ANOVA**analysis can create a problem. But for small variance differences,**ANOVA**can be very robust. - For the factorial
**ANOVA**, use can face heavy issues. - Now for the Excel. Things are a lot more complicated than it seems.
- For homogeneous types of data and equal cell size, Excel can execute the
**ANOVA**analysis quite easily. But if the data**samples**lack homogeneousness and have different cell sizes, then excel refuses to execute the**ANOVA**analysis. - It only can execute the
**ANOVA**analysis in two ways withou**t replication**provided that the rows are different numbers only. - In two ways with
**replication**, both the row and the column needs to be the same in the dataset. - This is because Excel is not efficient software for carrying out calculations with varied datasets. Variations of datasets make Excel throttle. Other software like the
**SPSS**/**R**could easily deal with this type of situation.

## Things You Should Keep in Mind

- Excel’s
**ANOVA**tool works perfectly whether we input the correct data or risk incorrect data. - To acquire an exact
**F Value**, ensure the initial variance is less than the second variance.

**Download Practice Workbook**

Download this practice workbook below.

## Conclusion

To sum it up, the issue of how you can do a two-way **ANOVA** analysis in Excel with an unequal sample size in Excel is answered here with 2 different examples with elaborate explanations.

For this problem, a workbook is available for download where you can practice these methods.

Feel free to ask any questions or feedback through the comment section.

## Related Articles

- How to Interpret Two-Way ANOVA Results in Excel
- How to Do Repeated Measures ANOVA in Excel
- How to Apply Rows Per Sample ANOVA in Excel
- Randomized Block Design ANOVA in Excel
- How to Do One Way ANOVA in Excel

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